Matthew L. Rise

	Matthew L. Rise Shaw Assistant Scientist Phone: (414) 382-1734 Fax: (414) 382-1705 E-Mail: Great Lakes WATER Institute 600 E. Greenfield Ave. Milwaukee, WI 53204

Research Interests For the past few years, I have helped develop genomic resources (e.g. EST databases, cDNA and oligonucleotide microarrays) for salmonid research. DNA microarrays allow the researcher to analyze relative expression levels of thousands of genes simultaneously. I have used these tools to study variability of gene expression within and between salmonid species, transcriptome dynamics over rainbow trout embryogenesis, and the responses of salmonid transcriptomes to various insults (e.g. rickettsial infection, pulp mill effluent exposure). Currently in the lab, these genomic resources are being used to identify embryonic trout molecular biomarkers of organophosphate pesticide (OP) exposure. OPs exert their toxic effects by inhibiting acetylcholinesterase, an enzyme critical to the normal functioning of cholinergic synapses. In my lab, various techniques are used to assess the impact of OP exposure on salmonid morphogenesis and behavior. Other potential stressors under similar evaluation in the lab include heavy metals, various anthropogenic effluents (and individual contaminants therein), and diets of varying nutritional quality. We further develop salmonids as models for studying the effects of environmental toxins on human health.

Education Whitworth College, BS Biology, 1988 Boston College, MS Molecular Genetics, 1990 University of Victoria, PhD Developmental Biology, 2001 Centre for Biomedical Research, U of Victoria, PDF Genomics, 2003

Selected Publications von Schalburg KR, McCarthy SP, Rise ML, Hutson JC, Davidson WS, and Koop BF. 2006. Expression of morphogenic genes in mature ovarian and testicular tissues: Potential stem-cell niche markers and patterning factors. Molecular Reproduction and Development 73:142-152. Ng SH, Artieri CG, Bosdet IE, Chiu R, Danzmann RG, Davidson WS, Ferguson MM, Fjell CD, Hoyheim B, Jones SJ, de Jong PJ, Koop BF, Krzywinski MI, Lubieniecki K, Marra MA, Mitchell LA, Mathewson C, Osoegawa K, Parisotto SE, Phillips RB, Rise ML, von Schalburg KR, Schein JE, Shin H, Siddiqui A, Thorsen J, Wye N, Yang G, and Zhu B. 2005. A physical map of the genome of Atlantic salmon, Salmo salar. Genomics 86:396-404. von Schalburg KR, Rise ML, Cooper GA, Brown GD, Gibbs AR, Nelson CC, Davidson WS, and Koop BF. 2005. Fish and chips: various methodologies demonstrate utility of a 16,006-gene salmonid microarray. BMC Genomics 6:126. von Schalburg KR, Rise ML, Brown GD, Davidson WS, and Koop BF. 2005. A comprehensive survey of the genes involved in maturation and development of the rainbow trout ovary. Biology of Reproduction 72:687-699. Rise ML, Jones SRM, Brown GD, von Schalburg KR, Davidson WS, and Koop BF. 2004. Microarray analyses identify molecular biomarkers of Atlantic salmon macrophage and hematopoietic kidney response to Piscirickettsia salmonis infection. Physiological Genomics 20:21-35. Rise ML, von Schalburg KR, Brown GD, Mawer MA, Devlin RH, Kuipers N, Busby M, Beetz-Sargent M, Alberto R, Gibbs AR, Hunt P, Shukin R, Zeznik JA, Nelson C, Jones SRM, Smailus DE, Jones SJM, Schein JE, Marra MA, Butterfield YSN, Stott JM, Ng SHS, Davidson WS, and Koop BF. 2004. Development and application of a salmonid EST database and cDNA microarray: data mining and interspecific hybridization characteristics. Genome Research 14:478-490. Burke RD, Murray GA, Rise M, and Wang D. 2004. Integrins on eggs: the bC integrin subunit is essential for formation of the cortical actin cytoskeleton in sea urchin eggs. Developmental Biology 265:53-60. Rise M and Burke RD. 2002. SpADAM, a sea urchin ADAM, has conserved structure and expression. Mechanisms of Development 117:275-281. Murray G, Reed C, Marsden M, Rise M, Wang D and Burke RD. 2000. The alphaBbeta C integrin is expressed on the surface of the sea urchin egg and removed at fertilization. Developmental Biology 227:633-647. Rise ML, Frankel WF, Coffin JM, and Seyfried TN. 1991. Genes for epilepsy mapped in the mouse. Science 253:669-673.